BIOKONVERSI BAHAN BERLIGNOSELULOSA MENJADI BIOETANOL MENGGUNAKAN Aspergillus niger DAN Saccharomyces cereviciae
Abstract: This study aims to
determine the feseability of bioethanol production from lignocellulosic
material by using simulataneous saccharification and fermentation (SSF)
processes with Aspergillus niger dan Saccharomyces cereviciae. Three different
lignocellulosic materials namely sengon wood (Paraserianthes falcataria),
gmelina wood (Gmelina arborea), pinus wood and (Pinus merkusii) were pretreated
using kraft process to remove lignin content. Then, pulp was treated by using
SSF processes. SSF runs were performed in 500 ml fermentors using a total
slurry 200 ml. The substrate and nutrient media were autoclaved (121 oC and 20
minutes). The samples diluted to 2.5 % (w v-1) of total slurry was used as
substrate. The substrate was added with 10 % (v v-1) A. niger (6.5 x 107 CFU
cc-1) of total slurry and then inoculated with 10 % (v v-1) yeast S. cereviciae
(1.5 x 109 CFU cc-1). The SSF experiments were run for 96 hours and the data
were investigated periodically every 24 hours. The results showed that total of
sugar and reducing sugar tended to decrease with time of inoculation whereas
ethanol concentration increase significantly. The growth of A. niger and yeast
S. cereviciae tended to incease in initial inoculation and decrease by the end
of inoculation. The bioethanol concentration on sengon, gmelina, and pinus were
0.53, 0.45 and 0.31 % respectively and produced yields 3.61, 4.60 and 4.16%
respectively.
Key words: bioethanol,
simultaneous saccharification and fermentation, Aspergillus niger,
Saccharomyces cereviciae
Penulis: Muhammad Daud, Wasrin
Safii, Khaswar Syamsu
Kode Jurnal: jpkehutanandd120070