Penentuan Kadar Rubraxanton pada Ekstrak Kulit Batang Garcinia spp
Abstract: In this study a High
Performance Liquid Chromatography (HPLC) has been used for determination of
rubraxanton on bark extracts Garcinia spp (Garcinia mangostana, Garcinia Cowa,
Garcinia griffitii, Garcinia dioica and Garcinia forbesii). HPLC system
consisted of C-18 reversed phase column with a length of 250 mm, diameter 4.6
mm, 20 mL injection volume, mobile phase methanol: water (gradient system with
polarity) and flow rate of 1ml/min. Rubraxanton levels obtained in this study;
9.161% for G.mangostana, 6.942% for G.cowa, 6.762% for G.dioica, 0.499% for
G.forbesii and 0.229% for G.griffitii. The method has been validated for
specificity, linearity, accuracy, precision, limits of detection (LOD) and
limits of quantitation (LOQ). The linearity of the method can be seen from the
regression coefficient r=0.9996 with a linearity range from 1.72 to 55 ug/ml.
Recovery of rubraxanton in the extract of G.mangostana was between 99.61 to
101.08%. Intra and inter day precision showed relatively small level of
standard deviation (lower than 2%). Limit Of Detection (LOD) and Limit Of
Quantitation (LOQ) are 0.55 ug/ml and 1.82 ug/ml respectively.
Penulis: Meri Susanti, Dachriyanus,
Deddy Prima Putra, Fatma Sriwahyuni
Kod Jurnal: jpfarmasidd130031
