Penetapan Kadar Alkaloid Ekstrak dari Etanolik Bunga Kembang Sepatu (Hibiscus rosa-sinensis L.)
Abstract: Kembang sepatu
flower (Hibiscus rosa-sinensis L.) was fractionally used as expectorant. Based
on Bioassay Guided fractionation, an active fraction was separated, and the
fraction was identified is Alkaloid was the major compound based on TLC
analysis. Viscosity value measured by viscometer was used as a Bioassay model
of expectorant activity in vitro and acetyl cysteine was used as positive
control. Alkaloid content determination of the ethanolic extract was measured
by TLC-Densitometric compared with standard curve of isolated alkaloid as the
selected marker (Y=12,1360X+2901,4474). The alkaloid content in the ethanolic
extract was determined as 2.35±0,67%.
Penulis: Mimiek Murrukmihadi,
Subagus Wahyuono, Marchaban, Sudibyo Martono
Kode Jurnal: jpfarmasidd130028
