ANALISA PENGARUH INTENSITAS SUMBER CAHAYA PENGINDUKSI FLUORESENSI TERHADAP INTENSITAS FLUORESENSI KLOROFIL PADA DAUN BAYAM MENGGUNAKAN METODE FLUORESCENCE IMAGING
Abstract: Chlorophyll
fluorescnce imaging and spectroscopy has been developed intensively during last
decade to detect early symptoms of plant diseases and effects of environtmental
stresses on plants. An economical, portable, and remote sensing system is
needed for those purposes. In this research, a fluorescence imaging system was
built using a LED with 465 nm wavelength, some neutral density filters and a 3
Mega Pixel CMOS camera. The intensities of LED light were varied using 5
different optical densities of the neutral density filters which were
represented by their optical power after filtered 5,0 mW; 3,3 mW; 2,0 mW; 1,5
mW; and 0,7 mW respectively. This system was used to investigate the relation
between the intensity of LEDs as a fluorescence inducer and the fluorescence
intensity of spinach leaves grown under two variations of sunlight intensity.
The variations were about 90% using plastic cover and 40% using plastic plus
paranet cover. The spinach plant was Amaranthus tricolar varitas. The
differences between chlorophyll fluorescence intensity of the spinach leaves
for both treatment were also investigated. The fluorescence intensities were
found from RGB plot using ImageJ software. The reseach results showed that
intensity of LED light influenced the fluorescence intensity resulted on the
spinach leaves. The higher the intensity of LED, the higher the fluorescence
intensity. The spinach leaves grown without paranet provided higher
fluorescence intensity however, the difference between both treatments was seen
higher when LED intensity was the lowest which was about 41,6% difference.
Keywords: Fluorescence
Imaging, ImageJ, CMOS camera, LED-Induced Fluorescence, Excitation Intensity
Variation
Penulis: Minarni, Iswanti
Sihaloho
Kode Jurnal: jpfisikadd150214
