ANALISA PENGARUH PANJANG GELOMBANG SUMBER CAHAYA PENGINDUKSI FLUORESENSI TERHADAP FLUORESENSI KLOROFIL PADA DAUN BAYAM YANG DIPENGARUHI VARIASI SINAR MATAHARI
Abstract: Chlorophyll
fluorescence imaging and spectroscopy can be used to detect early abnormalities
on plants, which are caused by plant diseases, harsh environments, and
intentional treatments. Development in chlorophyll imaging and spectroscopy
which is economical, low cost, and portable are needed in attempts to explore
fluorescence spectrum as markers for diseases and environmental stresses on
plants. In this research, a fluorescence imaging system was built using LEDs
with three variations in wavelengths i.e. 450 nm; 525 nm; and 680 nm, and a 3
Mega Pixel CMOS camera. The LED light was used as an excitation beam to induce
chlorophyll fluorescence of spinach leaves. Relation between the LED
wavelengths and the fluorescence intensities of the spinach leaves were
investigated. The samples were Spinach leaves from Amaranthus tricolor Spinach
plants grown under two variations of sunlight intensities. Two variations of
the intensities were about 90% using plastic as the cover and 40% using plastic
plus paranet. Relation between the sunlight intensity and fluorescence intensity
was also investigated. The fluorescence intensity of the leaves were measured
from RGB plot using Image-J software. The research results show that
fluorescence intensity of the samples without paranet were higher than those
using paranet. This is possibly caused by chlorophyll contents which is higher
on plants without paranet. The excitation wavelength that showed the highest
different fluorescence intensity was 680 nm, which showed 6,3% the difference
in fluorescence intensity using and without paranet while for other excitation
wavelength were 0,4% and 1,7% for 450 nm and 525 nm respectively.
Keywords: Amaranthus tricolor
spinach, chlorophyll fluorescence, fluorescence imaging, Image-J, wavelength
dependent fluorescence
Penulis: Fitria Asriani,
Minarni
Kode Jurnal: jpfisikadd150213