ANTI FUNGAL ACTIVITY OF METHANOLIC EXTRACT OF Usnea sp. AGAINST Malassezia furfur
ABSTRACT: The antifungal activity of methanolic extract of Usnea sp.
(MEU) against Malassezia
furfur ATCC 14521, in terms of in vitro susceptibility, minimum inhibitory
concentration (MIC) and minimum fungicidal concentrations (MFC) were
investigated using broth microdilution method with endpoint after 48 h.
Time-kill curves were determined at concentrations 0×, 1/2×, 1×, 2×, 4× and
8×MIC. MEU was susceptible against M. furfur with diameter clear zone of 34 mm
for 1 mg/ml. MIC and MFC values were 16 μg/ml and 64 μg/ml, respectively.
Time-kill curve demonstrated that treatment with 4×MIC (64 μg/ml) and 8×MIC
(128 μg/ml) of MEU for 4 h and 1 h, respectively, was able to kill 100 % of M.
furfur. MEU shows potential as an antifungal agent for inhibiting the growth of
M. furfur ATCC 14521 in vitro. MEU might be a useful alternative for treating
dandruff, tinea versicolor, tinea capitis, and seborrhoeic dermatitis due to
the growth inhibition of M. furfur.
Author: Rukayadi. Y, Diantini.
A, and Lestari K
Journal Code: jpbiologigg120007
