DESIGN OF ELECTROPHORESIS DEVICE FOR OPTIMATION OF DNA VISUALIZATION AND DNA CONCENTRATION USING SOFTWARE
ABSTRACT: Molecules of
deoxyribo nucleic acid (DNA) show a strong polarization allowing for both
motions of the dielectrophoresis induced by polarization and electrophoresis
based on its negative charge. Considering high subjective and less quantifiable
result of the visualization based qualitative test of DNA on gel
electrophoresis, designing the tool using a combination of the principles of
electrophoresis and dielectrophoresis completed with a software for
optimization of DNA visualization and to measure the concentration of small and
large–sized DNA fragment is very needed. Accuracy of measurement of DNA
concentration using a spectrophotometer UV /VIS is depend on its availability
in the laboratory. The aim of this study was to design device for optimization of
DNA visualization and measuring the concentration
in the gel electrophoresis using MatLab-
based software. Experiment using this software measured the concentration of
DNA based on its visualization and compared it with calculation obtained from
spectrophotometer UV/VIS. The research results showed that the amount of DNA
analysed using a spectrophotometer tend to similar with the measurement results
using the MatLab-based software although there was differences in quantitative
values.
Author: H.P. Kusumaningrum, W.
S. Budi, M. Azam, A. Bawono
Journal Code: jpfisikagg140017
