MOLECULAR CHARACTERISATION of Polymesoda erosa (Solander, 1786) INHABIT TWO DIFFERENT HABITATS

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ABSTRACT: The  first  organismic  response  on  pollution  is detoxification  through  physiological  processes. On molecular level, pollutant causes chromosome damage and DNA mutation. DNA mutation might lead to a loss or form of restriction sites. Therefore, it is possible to detect pollution using PCR-RFLP marker such as using cytochrome c oxidase I gene.  This study used survey method by applying purposive random sampling. The samples were collected during the field trip in April 2011 at Donan River and Segara Anakan Cilacap.  Ten restriction enzymes were used. Selected enzymes, mutation event, and molecular characters were defined descriptively based on the appearance of restriction fragment on agarose gel. Genetic diversity h was estimated with the help of Arlequin software version 2.0. The result showed that  Alu1  and  Vsp1  could cut the PCR product, heavy plumbum (Pb) pollution on sediment caused nucleotide mutation on cytocrhome c oxidase 1 gene, cytochrome c oxidase gene 1 can be used as molecular character to differentiate Polymesoda erosa  inhabit heavily and less plumbum polluted ecosystem, and generally  Polymesoda erosa  population at Segara Anakan  showed  high  genetic  diversity.  However, if we come into detail on each sampling site, some showed low genetic diversity while other sites showed high genetic diversity.
Key words: Pollution, Polymesoda erosa, cytochrome c oxidase 1, PCR RFLP, restriction enzyme
Author: Nuryanto, A. and Sastranegara. M.H.
Journal Code: jpbiologigg130004

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