Application of Aquaculture Natural Food Produce by Protoplast Fusion Process of Dunaliella salina and Phaffia rhodozyma

Abstract: Recently, fishponder income tend to be static so that required an effort to accurately and efficiently one of them through  aquacultures    food  diversification.  So  far,  efforts  to  observe  the  pattern  of  introduction  of  a  new  feed, applications and their impact on aquaculture and fish farmers income has never been measured. Diversification of feed use protoplast fusion process from D. salina and P. rhodozyma which rich in natural carotenoids is needed in aquaculture because they will increase survival and body weight of animal farming. Different types of carotenoids can be combined through a process of protoplasm fusion making it cheaper, faster and efficient for diversification and development  of  carotenoid-rich  diet.  The  specific  objectives  of  this  research  is  the  development  of  natural  food aquaculture  with  high  carotenoid  content  using  protoplasm  fusion    from  the  microalgae  D.  salina  and  yeast  P. rhodozyma. The early stage in this research will cultivate recombinant followed by quantitative analysis of carotenoid production compared  with  controls.  The  second  stage  is  the  food  diversification  with  different  concentrations of recombinant and comparisons with commercial food followed by cost-benefit analysis. The research results have been  obtained  which    most  carotenoid-rich  natural  food  resulted  from  recombinant  fusion  of  D.  salina  and  P. rhodozyma. Its application in vitro have increased body weight of shrimp comparing to artificial feed. In addition, recombinant food showing stable growth in both freshwater and salt water, can breed naturally and are safe for animal aquaculture consumption and also the environment.
Key words: carotenoid, protoplast fusion, D. salina,  P. rhodozyma, recombinant
Author: Hersugondo, Hermin Pancasakti Kusumaningrum, Muhammad Zainuri
Journal Code: jpperikanangg100008

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