APPLICATION OF CLOVE OIL AS ANESTHETIC FOR SEA BASS (Lates calcarifer Bloch)
Abstract: The objective of
this study was to determine the safety and efficacy of clove oil as an anesthetic
in sea bass (Lates calcarifer) and the
potential application of clove oil as anesthetic to facilitate the sea bass fry
transportation. Acute toxicity test indicated the 24-hr LC50 value of clove oil
in sea bass fry as 30 ppm with slope function of 1.079 (1.05 to 1.107). In
efficacy test, fish were exposed to 5, 10, 15, 20 and 25 ppm of clove oil for
15 minutes. At 5 ppm, clove oil caused only sedation effect (partial loss of
reaction to external stimuli) while at 20 ppm, fish entered anesthesia stage
(failure to respond to external stimuli) within about 3 minutes. Fish recovered
from a 15-min period of exposure in 20 ppm clove oil within less than 10
minutes following removal from the anesthetic solution. There was neither
mortality nor abnormal behavior of fish during 15-min exposure of clove oil as
well as during 7 days post recovery from anesthesia. The potential application
of clove oil as an aid in the transport of sea bass fry in plastic bag was also
investigated. At 5 ppm, clove oil could reduce activities of the fish without
loss of equilibrium (sedation stage) during the 4 hour simulated transport at
50 fish per 1,000 ml sea water (15 ppt). At 20 ppm, clove oil caused loss of equilibrium
in fish resulting in the anesthesia stage throughout the 4 hour period.
However, there was no improvement on survival rate and fish behavior with the
use of clove oil during and after this 4 hour transport. Simulated transport at
50 fish per 500 ml sea water (15 ppt) for 8 hour did show better significant
survival rate with additional of 5 and 20 ppm clove oil. In both short and long
term transport study, clove oil did show the benefit by reducing the fish
activities judging from the reduction of oxygen consumption, ammonia and carbon
dioxide levels. Addition of appropriate concentration of clove oil in transport
water ensured that the fish would stay calm by reducing fish activity and
therefore, prevented any drastic changes of water qualities.
Author: Bambang Hanggono
Journal Code: jpperikanangg060005
Artikel Terkait :
Jp Perikanan gg 2006
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