BIOCHEMICAL GENETIC ANALYSIS OF THREE POPULATION OF MARBLE GROUPER, Epinephelus polypekhadion
Abstract: Genetic variations
of marble groupers, Epinephelus polypekhadion were evaluated to know genetic
performance of fish to support fish seed production. Fifty fish samples from three locations i.e. West Nusa
Tenggara, South Sulawesi and East Java were collected for analysis. Genetic
analysis has been examined through allozyme electrophoresis by using 11
enzymes (ADH, GPI, SDH, IDH, LDH, ME,
PGM, 6PGD, MPI, SP, EST). The result showed
that 16 loci were detected, and three of them were polymorphism enzymes namely Isocitric
dehydrogenase (IDH*), Glucose Phosphate Isomerase (GPI-1*) and Esterase (EST-2*).
One locus (EST-2) was polymorphic in the
East Java and West Nusa Tenggara populations and three loci (EST-2, GPI-1 and
IDH) were polymorphic in the South Sulawesi population. The heterozygosity
ranged from 0.007 to 0.034. Rogers genetic distance between population pairs
were ranged from 0.005 to 0.012 (average = 0.009). Differences between genetic
populations were significant (P<0.05). East Java and West Nusa Tenggara populations
have same gene pool.
Author: Gusti Ngurah Permana, Haryanti, Sari Budi Moria, Nyoman Adiasmara Giri, dan Ketut Sugama
Journal Code: jpperikanangg070003
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