Evaluasi Pertumbuhan dan Keragaman Genetik Tanaman Palahlar Gunung (Dipterocarpus retusus blume.) dan Palahlar (Dipterocarpus hasseltii blume.) Berdasarkan Penanda RAPD

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Abstract: Dipterocarpus  hasseltii  (palahlar)  and  Dipterocarpus  retusus  (mountain  palahlar)  are  plant  species  o f Dipterocarpaceae family that originate from West Java which population is getting rare. Information about growth development  and  genetic  variation  of  palahlar  is  important  to  support  culturing  program  and  its  gene tic conservation. One of the techniques to study palahlar growth is through observation of the plant height and stem diameter increment. DNA analysis is use to gain information of palahlar genetic diversity. One of the DNA based molecular  marker  widely  used as  plant  genetic  marker  is RAPD.  There were  2  primary that  resulting  the  best amplifying quality in genetic diversity analysis that was OPO-13 dan OPY 20. Analysis result with POPGENE 32 was resulting average genetic diversity between classes as much as 0.1869. The biggest genetic diversity for palahlar was on the medium growth class as much as 0.2498 dan for mountain palahlar was in small growth class as much as 0.2240. Cluster analysis shows that there were 2 big groups that were medium-high growth class group and small  growth  class  group.  Dendrogram  shows  that  there  were  2  species  in  one  group,  that  was  palahlar and mountain palahlar that was in one group of small growth class. The closed genetic distance existed between medium growth class group of palahlar and high growth class group (0.0383), while the far distance is between palahlar of small growth class group with mountain palahlar of mediaum growth class group as much as 0.1826.
Keywords: palahlar, plant height, stem diameter, genetic variation, DNA
Penulis: Detty Sumiyati, Fifi Gus Dwiyati, Istomo, dan Iskandar Z Sirega
Kode Jurnal: jpkehutanandd090084

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Jp Kehutanan dd 2009