IDENTIFICATION AND CHARACTERIZATION OF CARMOVIRUS ON CARNATION (DIANTHUS CARYOPHYLLUS L.) IN WEST JAVA, INDONESIA
ABSTRACT: Carnation has a
highly economic demand of cut flower in Indonesia. Field observations in West Java Indonesia was
conducted in order to find the typical mottle symptoms that was a suspect
caused by a virus disease. Identification of the virus was respectively
conducted by performing ELISA test with four anti sera and characterizations
held by bioassay, observing of virion particles, detecting of nucleic acid by
RT-PCR and nucleotide sequencing. Total of 403 samples were collected from
plants with or no virus-like symptoms. Among those all tested, 83% were found
to be infected by Carnation mottle virus (CarMV), but negatively against
Carnation ringspot virus (CRSV), Carnation laten virus (CLV), and Carnation
vein mottle virus (CVMV) antisera. By mechanical inoculation, the virus was
able to infect systemically Cenopodium quinoa and locally infect on others.
However on Phalaenopsis sp and Gomprena globosa, there was symptompless found.
The isometric CarMV particles size was approximately 30 nm. RT-PCR using
specific primers of CP gene of CarMV successfully amplified a DNA sized 1000
bp. CarMV West Java Indonesian (Idn-WJ)
isolates possessed the highest nucleotide and amino acid homology with CarMV
from Spain and was in the same cluster with CarMV from China, Taiwan and
Israel.
Author: Erniawati Diningsih,
Gede Suastika, Tri Asmira Damayanti, Slamet Susanto
Journal Code: jppertaniangg150028
