Metode Perlakuan Pendahuluan untuk Penyimpanan Benih Melur pada Suhu Sangat Rendah Pretreatment Method of Melur Seeds Storing at Extreme Low Temperature
Abstract: Cryopreservation is
a method of long term storage in which an extremely low temperature of nitrogen
liquid (-196oC) being
used to preserve
seeds of recalcitrant
characteristics. The study
was aimed to determine
the standard protocol
of vitrivication in
melur (Podocarpus neriifolius)
seeds storing in various
cryoprotectans. The use
of sucrose PVS2
and DMSO as
a cryoprotectan were
employed to protect the
regularly-desiccated seeds during preserving in liquid nitrogen for 1 hour.
Evaluation was made on the
viability by germinating
the seeds in
green house. Randomized
completely design of 3 factors was
used in this
study. The results revealed that
seed moisture content
was 53% after being desiccated using
vaccuum-desicator for 24
hours and showed
more than 85%
of germination, in average. Vitrivicated seeds in DMSO for 2
hours performed 66.67% germination. Meanwhile, seeds in sucrose and PVS2 for 1
hour reached of 53.33% and 25.33% of germination, respectively. Preservation in
liquid nitrogen for 1 hour did not succeed to keep any treated seeds as there
was no germination found during
viability testing.
Penulis: Dida Syamsuwida dan
Aam Aminah
Kode Jurnal: jpkehutanandd080079
