Molecular Determination of a Green Algae Isolate to Detecting 1-Deoxy-D-Xylulose-5-phosphate Synthase (DXS) Gene in Improvement of Carotenoid Production
Abstract: Carotenoids
production levels are not yet competitive with carotenoid levels presently
produced by fermentation, synthesis and isolation. It needs application of
metabolic engineering and genetic engineeringtechniques in improving their
production. An attempt to optimize
carotenoid production from local isolate ofgreen algae from BBAP Jepara has
faced several problems, primarily related to the microbiological and
eco-physiological characteristic which affecting growth that have not
sufficiently been understood. A misnamed ofspecies also have arisen due to
wrong characterization. One local
isolate of an algal species from BBAP Jepara was found potentially useful as
source of carotenoids in food additives or as food supplement in fish farming.
It was suspected as representing a strain of Dunaliella. Previous studies to
improve carotenoid production using molecular approach on have shown unagreement. Therefore, the present study
aimed to determinate the species of green algae isolate from Jepara waters
based on molecular techniques using 23S rRNA approach for detecting DXS gene.
Molecular analysis by 23S rRNA alignment showed the close relationship among
isolate of green algae and most all of member of Cyanobacteria. Closest
similarities was showed by Cyanobacterium sp. MBIC 1021 with 99 % similarity and Synechocystis
PCC6308 with 95 % similarity. Synechocystis was the only member of
Cyanobacteria which have DXS gene. Multiples aligment sequences of partial DXS
gene on the conserve region among seven species confirmed this result. The DXS gene
analysis also showed closest relationship between partial DXS gene of
Cyanobacteria Synechocystis and a green algae isolate. The result of this
analysis proven as valuable parameter for the interpretation of the relation among
DXS gene of a green algae isolate and Cyanobacteria and increase the
possibility in getting the complete DXS gene from local isolate of green algae
by designing primers from DXS gene of Synechocystis as a member of
Cyanobcteria.
Author: Hermin Pancasakti
Kusumaningrum, Joedoro Soedarsono
Journal Code: jpperikanangg060001

Artikel Terkait :
Jp Perikanan gg 2006
- Shell Shape Variation of Tropical Limpet Cellana testudinaria (Class: Gastropoda, Family: Patellidae) Living on the Rocky Shore in Relation to Their Zonal Distribution
- Distribution of 90Sr in the High Seas and Coastal Regions of Korea-Japan-Russia-China
- The Influence of Temperature - Food Availability on the Tissue Growth of Sea Scallop Placopecten magellanicus
- THE EFFECT OF DIFFERENT LEVELS OF DIETARY N-3 FATTY ACID ON THE REPRODUCTIVE PERFORMANCE OF ZEBRAFISH (Brachydanio rerio)
- APPLICATION OF CLOVE OIL AS ANESTHETIC FOR SEA BASS (Lates calcarifer Bloch)
- The Sape Strait Cephalopod Resource and Its Response to Climate Variability
- Distribution, Abundance, and Biomass of Tropical Limpet Cellana testudinaria (Class: Gastropoda, Family: Patellidae) Living on the Rocky Shore of Ohoiwait, Southeast Moluccas, Indonesia
- Tracing Sewage Contamination in Urban Tropical Coastal Waters