OBTAINING ARTEMISIA CINA POLYPLOIDY THROUGH PLANT GROWTH REGULATOR TREATMENT IN SHOOT CULTURE
ABSTRACT: Artemisia cina is a
medicinal plant species which produces bioactive compound potential to
anti-tumor, antifungal and antibacterial medicines. This study was aimed to
obtain A. cina polyploid plants through a treatment of growth regulators in
shoot culture. The shoot were treated in 1; 15; 2; and 3 mg L-1 of 2,4-dichloro
phenoxy acetic acid (2,4-D) combined with 1; 1.5; 2; 3 mg L-1 of Benzyladenine
(BA) for 28 days. Chromosome analysis showed that the highest polyploidy
percentage, 23%, was reached in the treatment of 2 mg L-1 of 2,4-D combined
with 1 of BA mg L-1. The polyploidy level varied, comprising 2n=3x, 2n=4x,
2n=5x, 2n=6x, with the highest polyploidy level percentage, 28.57%, and it was
attained in the tetraploid (2n=4x). Polyploid plants had larger leaves area,
larger stomatal size, and higher chlorophyll content than diploid plants.
However stomatal density of polyploidy plants was lower than that of in diploid
plants.
Author: Marina Herawati,
Endang Pudjihartati, Suwijiyo Pramono, Endang Sulistyaningsih, Aziz Purwantoro
Journal Code: jppertaniangg150017
