PENCIRIAN MANANASE Streptomyces costaricanus 451-3

ABSTRACT: CHARACTERIZATION OF Streptomyces costaricanus 45I-3 MANNANASE Major component of hemicelluloses are mannans (softwoods) and xylans (hardwoods). Hemicclluloses arc used by microbes as a  carbon sources.  Mannanase and xylanasc arc enzyme complex that  are able to  degrade hemicelluloses. Mannanase activity from Streptomyces costarianus 451-3 was tested in locust bean gum 0.5% and coconut meal 0.5'\1, medium and was detected by dinitrosalysilic acid method. Protein concentration was measured using Bradford method. Mannanase and xylanase activity were also detected using birchwood xylan and oatspelt x~·lan medium. The optimum temperature and pH of Streptomyces mannanase strain 451-3 was 40 "C and 6,0, respectively. The addition lmM of Mg2+ and Zn2+ at final concentration increased the mannanase activity for about 30% and 80'Yo, while lmM Mnl+, Ca2+ and Co2+ decreased its activity for about 67%, 100°/.,, and 60%, respectively.  The addition of I mM ethylene diamine tetraacetic acid tend to decreased the enzyme activity to 30%. The medium which contain birchwood xylan dan oatspelt xylan could induce mannanase activity, hut in a lower degree then that of xylanase.
Keywords: mananase, streptomyces, xilanasc
Penulis: Anja Meryandini IJ,  Dwi Ambarawati, Nisa Rachmania
Kode Jurnal: jppertaniandd080106

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Jp Pertanian dd 2008