PENCIRIAN MANANASE Streptomyces costaricanus 451-3
ABSTRACT: CHARACTERIZATION OF
Streptomyces costaricanus 45I-3 MANNANASE Major component of hemicelluloses are
mannans (softwoods) and xylans (hardwoods). Hemicclluloses arc used by microbes
as a carbon sources. Mannanase and xylanasc arc enzyme complex
that are able to degrade hemicelluloses. Mannanase activity
from Streptomyces costarianus 451-3 was tested in locust bean gum 0.5% and coconut
meal 0.5'\1, medium and was detected by dinitrosalysilic acid method. Protein
concentration was measured using Bradford method. Mannanase and xylanase
activity were also detected using birchwood xylan and oatspelt x~·lan medium.
The optimum temperature and pH of Streptomyces mannanase strain 451-3 was 40
"C and 6,0, respectively. The addition lmM of Mg2+ and Zn2+ at final
concentration increased the mannanase activity for about 30% and 80'Yo, while
lmM Mnl+, Ca2+ and Co2+ decreased its activity for about 67%, 100°/.,, and 60%,
respectively. The addition of I mM
ethylene diamine tetraacetic acid tend to decreased the enzyme activity to 30%.
The medium which contain birchwood xylan dan oatspelt xylan could induce
mannanase activity, hut in a lower degree then that of xylanase.
Penulis: Anja Meryandini IJ, Dwi Ambarawati, Nisa Rachmania
Kode Jurnal: jppertaniandd080106
