SPECIES DIFFERENTIATION OF FISH SAMPLES BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF CYTOCHROME B GENE

ABSTRAK: Metode  pengukuran  polimorfisme  fragmen  hasil  pemotongan  produk reaksi  polimorfik  berantai    oleh  enzim  restriksi  spesifik  (polymerase  chain reaction-restriction  fragment  length  polymorphism,  RFLP-PCR)  telah  digunakan untuk membedakan beberapa jenis ikan mentah. Situs cytochrome b mitokondria, yang  diamplifikasi  oleh  primer  universal,  dipotong  menggunakan  empat  enzim restriksi (Bfa I, Hinf I, Msp I, Mbo II) sehingga dapat dianalisa fragment-fragment pendeknya.  Hasil  yang  diperolah  dari  pemotongan  oleh  enzim  restriksi  tersebut ternyata  dapat  digunakan  untuk  membedakan  tiap  jenis  ikan  sampel.  Hasil penelitian ini menunjukkan bahwa PCR dan RFLP-PCR merupakan metode yang sensitif  dan  dapat  dilakukan  dalam  waktu  singkat  untuk  membedakan  berbagai jenis ikan mentah.

Key Word: Polimorfisme fragmen, cytocrome b gene

Author: Haryani Y, Tunung R, Zuraini M I, and Son R

Journal Code: jpperikanangg090009

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