THE EFFECT OF DIFFERENT LEVELS OF DIETARY N-3 FATTY ACID ON THE REPRODUCTIVE PERFORMANCE OF ZEBRAFISH (Brachydanio rerio)

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Abstract: This experiment was conducted to determine the dietary n-3 fatty acid requirement for reproduction of broodstock zebrafish,  Brachydanio rerio.  Three isonitrogenous (39% crude protein) and isocaloric (3,260 kcal digestible energy/kg diet) practical diets, namely diets A, B, and C, with different levels of fatty acids were fed to zebrafish broodstock.  The broodstock were cultivated in aquaria.  Diet A contained low dosage of n-3 fatty acids (0.4%) and 2% n-6 fatty acids, while diets B and  C contained 2% n-6 fatty acids, combined respectively with 1% and 1.5% n-3 fatty acids.  Fish were fed ad satiation for 60 days using these diets.  During feeding periode, gonade maturation stages were examined.  The n-3 and n-6 fatty acids affected fecundity, fertilization rate, and hatching rate.  On the other hand, fish fed on diets A, B, or C did not show any significance differences in the gonade somatic index and total Survival Rate (SR) of larvae produced.  Fish fed on diet B produced the highest fecundity (616 eggs/g of fish), fertilization rate (94.6%), and hatching rate (93.8%).  The total lipid content of eggs were significant, ranging from B (29.7%), C (23.7%), and A (16.1%).  At a dosage of 2% n-6 fatty acids, zebrafish require 1% of dietary n-3 fatty acids in the diet for reproduction.  Excess dosage of n-3 fatty acid in the diet adversely affected fecundity, fertilization rate, and hatching rate.
Key words:  Brachydanio rerio, fatty acid, reproductive performance
Author: Nur Bambang Priyo Utomo, Ing Mokoginta, and Lia Nurmalia
Journal Code: jpperikanangg060006

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