Anti-inflammatory and analgesic effect of bromelain in mice and rats
ABSTRACT: Bromelain is a crude
aquaeous extract obtained from both the stem and fruit of the pineapple plant, which
contains a number of proteolytic enzymes. The analgesic and anti-inflammatory
activities of bromelain were investigated. Bromelain was evaluated for
anti-inflammatory effect by carrageen-induced rat paw edema. The analgesic
activity was tested by acetic acid-induced writhing response and hot plate
method in albino mice. Twenty adult male Balb/c mice (20-25 g) were used to
evaluate the analgesic effect. And 25 male Sprague-Dawley rats (200-250 g) were
used to study the anti-inflammatory activy. This study showed that bromelain in
doses of 10; 20 and 40 mg/kg showed 11.3; 45.1 and 56.3% inhibition of paw
edema respectively at the end of three hours, and the percentage of protection
from writhing was 11.1; 23.4 and 40.8% respectively. In the hot plate model,
the bromelain at 20 and 40 mg/kg increased the pain threshold significantly
after 30 min, 1, 2 and 4 h of administration. Bromelain showed dose-dependent
action in all the experimental models. These study suggest that bromelain has anti-inflammatory and
analgesic properties.
Author: Sri Agus Sudjarwo
Journal Code: jpkedokterangg050003
