Genotyping of hepatitis B virus from dried and stored serum on filter paper
Abstract: HBV genotype has
aclose association with prognosis and therapy as well as for epidemiology
study. However, this examination can be done only in large cities that are not
practical to send serum sample due to geographical burden and facilities. The
aim of this study is to know whether HBV genotype can be determined from dried
and stored serum on filter paper and compare the result with sera drawn
directly from chronic hepatitis B (CHB) and hepatoma patients. Twenty-three
serum samples were obtained from CHB patients. HBV DNA were quantitatively
determined with Cobas Amplicor HBM (Roche Diagnostics GmBH, Germany) and dropped
on to 3 x 1 cm filter papers. After allowed to dry in a plastic clip, it were
put in a closed envelope then stored for 1 week in room condition (27 – 33 oC).
DNA extraction were done from the filter papers after a short incubation period
and HBV genotypes were determined with PCR and specific primers. For
comparison, 20 CHB-Hbe(+) samples and 29 hepatoma samples were drawn directly
and not dried. HBV genotype were detected in 18/23 (78.2%) from dried serum
samples on filter paper while in sera that were not stored, from CHB-HBe(+)
samples, 20/20 (100%) could be determined while from hepatoma patients, 24/29
(82.7%) samples. The proportion of genotype were in line with other reported
HBV genotype examination for Indonesia. It is concluded that detection of HBV
genotype can be done from dried serum in filter paper and stored for 1 week.
Author: Rino A. Gani, A. D.
Handayu, Reny Mulatsih, L. A. Lesmana, Ali Sulaiman, Nurul Akbar
Journal Code: jpkedokterangg050138
Artikel Terkait :
Jp Kedokteran gg 2005
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