IN VITRO DRUG SENSITIVITY OF Trypanosoma evansi BANGKALAN ISOLATES
ABSTRACT: Only a limited
number of drugs is available for Trypanosoma infection treatment. In Indonesia
there are two chemoterapheutics (isometamidium and diminazene) used for
treatment. The isometamidium does not act as a trypanocide of Indonesia
isolates. However, the clinical action of diminazene to Trypanosoma has not
been reported yet. The objective of this research was to obtain trypanocide
concentration of Trypanosoma evansi Indonesia isolates by in vitro test. Drug
sensitivities of Trypanosoma evansi Bangkalan isolates modified using Minimum
Eagle Medium and HEPES medium were measured for diminazene aceturate and
suramin using in vitro incorporation test. In control group, trypanosome
inoculate was added as a placebo substance in microtiter plate containing
trypanosomes. Bloodstream forms were isolated from Wistar strain Rattus
norvegicus, incubated at room temperature in serial drug dilutions in a
microtiter plate by adding 5 % of CO2. After 24 hours, the trypanosomes were
counted by inverted microscope to determine trypanocide action. The two
subjects research were different trypanocide action (p<0.05). The minimum
concentration with trypanocide action of diminazene aceturate was found to be
8.480 µg.ml-1.The minimum concentration with trypanocide action of suramin was
6.600 µg.ml-1
Author: Mochamad Lazuardi
Journal Code: jpkedokterangg050031
