IN VIVO GENE THERAPY WITH PLASMID DNA-TUBEROUS SCLEROSIS COMPLEX-2 (TSC-2) IN AN ORAL SQUAMOUS CELL CARCINOMA
ABSTRACT: Tuberous sclerosis
complex (TSC) is an inherited syndrome in which affected individuals are at
increased risk for developing benign tumors. Mutation of the TSC-2 gene
encoding tuberin on chromo-some 16p13.3 give rise to the clinical disorder of
tuberous sclerosis characterized by the development of hamartomas.
Objectives: In the present study, I conducted to examine whether
overexpression of TSC-2 can affect the growth of human oral squamous cell
carcinoma (OSCC) which have different expression level of p27K'P'. Methods: I
constructed an expression vector containing sense-oriented rat TSC-2 cDNA with
pcDNA3.1 (Invitrogen), and transfected to oral squamous cell carcinoma (B88) to
regulate the expression of TSC-2 gene in each transfectant. Western blot
analysis was carried out to determine the protein level of TSC-2. Therefore,
Tumor volume and body weight of nude mice was examined using tumorigenicity
assay.
Results: Overexpression of TSC-2 exerted the growth inhibitory effect of
oral squamous cell carcinoma. Sense-oriented B88-TSC-2 cancer cells, have high
expression of p27K'P1. Moreover, tumor induced by TSC2 up-regulated
transfectans become much smaller than those of control cells.
Conclusion: overexpression of TSC-2 may exert the antitumor effect on
oral squamous cell carcinoma through p27K'P' induction.
Author: Supriatno
Journal Code: jpkedokterangg050012
