SPERMATOZOA MOTILITY AND MORPHOLOGICAL RECOVERY PROCESS IN MICE (Mus musculus) AFTER THE INDUCTION OF 2-METHOXYETHANOL

Edit
ABSTRACT: This study used 30 male BALB/C mice aged 8 - 9 weeks with bodyweight of 28 - 30 grams. These experimental animals were divided into 6 groups, each comprising 5 mice. Group K1 was given with 2-ME of 200 mg/kg intraperitoneally every day for three weeks. The groups P1, P2, P3 and P4  were given with 2-ME, also for three weeks, and the administration was subsequently ceased for 1, 2, 3, and 4 weeks, respectively.  Group K0 (control) was injected with saline solution. Spermatozoa were taken from epididymal cauda. Observation was carried out for the motility of 100 spermatozoa with types A, B, C or D (WHO, 1992), as well as to normal and abnormal morphology in head and tail, and combination of head and tail. Data were tested using one-way variant analysis. Results showed that the administration of 2-ME every day for 3 weeks resulted in azoospermia, so that sperm motility and morphology was not observable. However, by  providing recovery period from 1 to 4 weeks, sperm motility  and morphology was found to recover into normal. There was a significant difference in sperm motility and morphology after treatment and recovery. As conclusions, 2-Methoxyethanol given to male mice for 3 weeks may result in azoospermia, and the effect of the length of cessation period of 2-ME administration increases the recovery of sperm morphology and motility.  
Keywords: 2-methoxyethanol, sperm motility and morphology
Author: Alfiah Hayati, Any Devi Rahmaninta, and IB Rai Pidada
Journal Code: jpkedokterangg050016

Artikel Terkait :

Jp Kedokteran gg 2005