SPERMATOZOA MOTILITY AND MORPHOLOGICAL RECOVERY PROCESS IN MICE (Mus musculus) AFTER THE INDUCTION OF 2-METHOXYETHANOL
ABSTRACT: This study used 30
male BALB/C mice aged 8 - 9 weeks with bodyweight of 28 - 30 grams. These
experimental animals were divided into 6 groups, each comprising 5 mice. Group
K1 was given with 2-ME of 200 mg/kg intraperitoneally every day for three
weeks. The groups P1, P2, P3 and P4 were
given with 2-ME, also for three weeks, and the administration was subsequently
ceased for 1, 2, 3, and 4 weeks, respectively.
Group K0 (control) was injected with saline solution. Spermatozoa were
taken from epididymal cauda. Observation was carried out for the motility of
100 spermatozoa with types A, B, C or D (WHO, 1992), as well as to normal and
abnormal morphology in head and tail, and combination of head and tail. Data
were tested using one-way variant analysis. Results showed that the administration
of 2-ME every day for 3 weeks resulted in azoospermia, so that sperm motility
and morphology was not observable. However, by
providing recovery period from 1 to 4 weeks, sperm motility and morphology was found to recover into
normal. There was a significant difference in sperm motility and morphology
after treatment and recovery. As conclusions, 2-Methoxyethanol given to male
mice for 3 weeks may result in azoospermia, and the effect of the length of
cessation period of 2-ME administration increases the recovery of sperm
morphology and motility.
Author: Alfiah Hayati, Any
Devi Rahmaninta, and IB Rai Pidada
Journal Code: jpkedokterangg050016
