T-lineage blast crisis of chronic myelogenous leukemia: simple record of 4 cases

Abstract: Blast crisis (BC) transformation in chronic myelogenous leukemia (CML) can involve each differentiation lineage of the hematopoietic system, i.e. granulocyte, monocyte, erythrocyte, megakaryocyte, and lymphocyte lineage. The lymphoid blast crisis (BC) leukemia cells usually belong to B-lineage, commonly having the phenotype of Pre-B stage of the B-lineage, in which cell-surface immunoglobulin (sIg) is not yet expressed. In contrast, T-lineage BC of CML is extremely rare. The objective of this study is to describe the fenotype, fusion transcript of bcr-abl, TdT, and cytoplasmic CD3 in T-lineage BC CML cases. Case report study. This report shows a simple summary of 4 cases of T-lineage BC of CML which have been collected in the phenotypic and genotypic analysis study for 17 years (1987-2004). In all cases, the chromosomal analysis revealed the presence of t(9;22)(q34;q11) at presentation. Cell surface analysis were done at diagnosis. Cases’ mononuclear cells stored as 10% DMSO were retrieved to be performed reverse transcription (RT) PCR BCR-ABL multiplex to demonstrate the presence of the fusion transcript of bcr-abl. RT-PCR was also performed for detecting the expression of cytoplasmic CD3ε and terminal deoxynucleotydil transferase (TdT). The results of cell surface antigen (CSA) at presentation showed that 1 case was CD7+, CD5-, and CD2-; 1 case CD7+, CD5+, and CD2-; and 2 cases CD7+, CD5+ and CD2+ indicating that all these T-lineage BC of CML cells show the phenotype of pre-(pro-) thymic stage phenotype. In the present study, two cases showed b2a2, one e1a2, and one negative bcr-abl transcript. The RT-PCR revealed the presence of CD3ε mRNA in all cases, and TdT mRNA in only one case. These results can constitute a basis for the future analysis of T-lineage BC of CML from now on.

Keywords: chronic myelogenous leukemia (CML), blastic crisis (BC), T-lineage, bcr-abl fusion gene, CDε, TdT

Author: Kartika W. Taroeno-Hariadi, Eiji Tatsumi, Mardiah S. Hardianti, Kaho Furuta, Meilani Syampurnawati, Bambang Karsono, Takayuki Ishikawa, Hiroki Yabe, Ken-Ichi Nagai, Takayuki Takahashi, Yuji Kishimoto

Journal Code: jpkedokterangg050129

Artikel Terkait :

Jp Kedokteran gg 2005

• Treatment of taeniasis and cysticercosis with praziquantel and albendazole
• The benefits of progesterone therapy in imminent abortion
• PM10 as Predictor of Ventilation Efficiency of Houses in Relation to Health Effect
• Fatty acids intake among diverse ethnic groups in Indonesia
• The effects of balanced low calorie diet on body composition and serum leptin of obese women
• QT dispersion, a simple tool to predict ventricular tachyarrhythmias and/or sudden cardiac death after myocardial infarction
• Endoscopical appearances of nonsteroidal anti inflammatory drug (NSAID)- enteropathy
• Role of general practitioner in the management of acute myocardial infarction
• Genotyping of hepatitis B virus from dried and stored serum on filter paper
• Development of bioassay for pathogenecity testing of Ureaplasma urealyticum as part of host-pathogen communication
• Detection of GAD65 autoantibodies of type-1 diabetes using anti-GAD65-abs reagent produced from bovine brain tissue
• The use of ceftriaxone impregnated beads in the management of chronic osteomyelitis
• The correlation between total lymphocyte count and the number of candida colony from the oral cavity in HIV/AIDS patients
• Survival rate and prognostic factors in advanced cervical cancer patients accompanied by renal impairment
• Left ventricular diastolic dysfunction in type 2 diabetes mellitus patient without cardiovascular disease: the association with microalbuminuria
• Hormonal contraception as a risk factor for obesity
• Effect of irrigating fluid temperature on core body temperature during transurethral resection of the prostate
• Intracranial germ cell tumour with extra cranial extension in a four month old infant – a case report
• Drospirinone: new generation of progestogen
• Implementation of 25-well culture plates for M. tuberculosis drug susceptibility testing in Indonesia
• Cytosolic estrogen and progesterone receptor content in the endometriotic tissues and endometrium in women with and without endometriosis
• Nutritional status of hyperlipidemics elderly in Indonesia according to body mass lndex (study in four Indonesian big cities)
• Age, duration of work, noise and vibration in inducing hearing and balance impairments
• Presurgical cytologic diagnostic test of uterine cavity in ovarian malignant tumor