THE DETECTION OF Staphylococcus aureus IN SWIFTLETS' NEST USING IMMUNOHISTOCHEMISTRY (STREPTAVIDIN BIOTIN)
ABSTRACT: A study to detect
the presence of Staphylococcus aureus in swiftlets' nest using immunohistochemistry
(Streptavidin biotin Complex) has been successfully done. Tissue and
supernatant were made from the nest, and the presence of the bacteria
Staphylococcus aureus was detected by means of immunohistochemical method. As
positive control, we used Staphylococcus aureus culture, while for negative
control we replaced Staphylococcus
aureus monoclonal antibody with Phospat Buffer Saline (PBS). The result showed
that staining with Staphylococcus aureus monoclonal antibody in swiftlets' nest
tissue revealed the presence of Staphylococcus aureus as a brownish group or
cluster, resulting from the reaction of enzymes and chromogen in Streptavidin
Biotin Complex. Based on this study, it can be concluded that immunohistochemical
method (Streptavidin Biotin Complex) can be used to detect the presence of Staphylococcus aureus in swiftlets' nest.
Author: Retno Oktorina,
Soedarmanto Indarjulianto, Sitarina Widyarini, Hastari Wuryastuti, R. Wasito
Journal Code: jpkedokterangg050038
