THE EFFECT OF 5α REDUCTASE INHIBITOR AND ESTROGEN IN PROSTATE PROLIFERATION: An Experimental Study in Rats
ABSTRACT: This was an
experimental study using posttest control group design involving Wistar strain
Rattus norwegicus as experimental animal. The purpose of this study was to
explain the mechanism of BPH in elderly. Samples were randomly divided into 2
groups, 1- and 2-month group, each comprising 26 rats. Each group was divided
further into two subgroups, one group received combined estrogen and
finasteride, and the other, receiving finasteride only, served as control
group. Each subgroup consisted of 13 rats. After treatment for 1 and 2 months,
the prostate was removed and examined for TGF-ß1, EGF, FGF, and proliferation.
Immunohistochemistry was used for examining TGF-ß1, EGF, FGF, and the
examination of proliferation was carried out using graticulae. This study
employed univariate analysis with 2 sample t test as TGF-ß1, EGF, and FGF had
no correlation. Data analysis used in this research was univariate analysis
with 2 sample t test. Analysis result showed that estrogen could reduce TGF-ß1
significantly in 1 month and 2 month groups (p < 0.05) and estrogen also
stimulated significant increase of EGF in 2 month groups (p < 0.05). Estrogen
also increased proliferation significantly in both 1 and 2 month groups (p <
0.05) but estrogen did not increase FGF significantly in both groups. Multiple
regression analysis on the effect of
TGF-ß1, EGF, FGF and estrogen to proliferation revealed that only TGF-ß1
had negative feedback. This indicated that TGF-ß1 decreased, so that the
proliferation increased. Estrogen had positive impact in proliferation,
indicating that increased estrogen would also increase proliferation. In
conclusion, estrogen increased the
proliferation of the prostate cell and EGF significantly and decreased the
expression of TGF-ß1 significantly. This leads to inhibition of proliferation,
and finally may cause the occurrence of BPH.
Author: Soetojo, Doddy M
Soebadi, Hendromartono, I Ketut Sudiana
Journal Code: jpkedokterangg050041
