Isolasi dan Identifikasi Kapang Saprofitik pada Sampel Tanah di Sekitar Kawasan Gunung Gamalama, Ternate
Abstrak: Microorganisms, such
as mould and bacteria, have important roles in organic matter decomposition;
and this process is fundamental to maintain soil structure and fertility.
Moulds have hypha with filament structures that can penetrate to the
substrates. Their enzymatic capability is higher than that of bacteria,
especially in decomposing organic compounds like lignin and cellulose. The
objective of the study was to isolate and identify saprophytic mould inhabiting
soil of Mount Gamalama, Ternate, North Moluccas. The mould isolation was based
on sample dillution method with Rose Bengal Cloramphenicol Agar isolation
media. The abundance of saprophytic mould counted by measuring the average
Colony Forming Unit (CFU)/ml of all mould colonies which grown on isolated
media by Total Plate Count (TPC) method. The diversity of isolated mould was
identified based on phenotypic characters by observing both macroscopic and
microscopic morphology. The result showed that the average of mould colonies
were between 13,30 x104 and 78,15 x104 CFU/ml. Identification based on
morphology from selected mould isolates showed that 25 fungal taxa and two
groups of unidentified fungal isolates were identified.
Penulis: Muhammad Ilyas
Kode Jurnal: jpbiologidd100172
