Perancangan Primer Oligonukleotida untuk Polimerisasi in Vitro Gen Sukrosa Sintase
Abstrak: The most important
problems in using polymerase chain reaction (PCR) are the efficiency of energy,
cost and time due to gene amplification. Oligonucleotide primer design of
sucrose synthase gene was conducted as a model of preliminary experiment to
amplify gene using PCR. In plant cells, this gene plays an important role in
carbohydrate metabolism, a sucrose molecule break down into glucose. This
design involved some computer software as bioinformatics tools. Five data
sequences of legumes were downloaded from gene bank using accession number of
AF030231, AJ311496, X92378, X69773, and D10266 belongs to soybean, pea, alnus
bean, fava bean, and mung bean, respectively. After sequences alignment, some
conservative regions were determined as the basis to construct forward and
reverse primer candidates. Furthermore, the candidates were tested for
compatibility. The results showed that the oligonucleotide primers can amplify
sucrose synthase gene with ± 1462 bp fragment size using 5’-AACTTTgTgCTTgA-3’
and 5’-TCCTTTgACTCCTTC-3’ for forward and reverse primer, respectively. Even
the PCR process weren’t applied, those primers might be universal primers to
amplify sucrose synthase gene of legume plants.
Penulis: Pieter Agustinus
Riupassa
Kode Jurnal: jpbiologidd090079
