ANALISIS RAPD KECIPIR POLONG PANJANG Psophocarpus tetragonolobus (L.) DC HASIL MUTASI IRADIASI SINAR GAMMA
ABSTRACT: Winged bean
[Psophocarpus tetragonolobus (L.) DC] is a tropical plant that has some
benefits and is very suitable to be cultivated in Indonesia. Study about
diversity of winged bean is very important to support the future development of
winged bean. Increased genetic diversity can be done through mutation. Gamma
ray is often used for inducing mutations. One of way to observe genetic
diversity resulted by molecular mutation is using RAPD method. The purpose of
this study was to identify the genetic diversity of winged bean resulted by gamma
ray irradiation. The method used in this study was an experiment by using RAPD
technique. Samples used were plant leaves that had been induced by gamma ray
with wavelength of 20 Gy, 25 Gy, and control plants that were not induced by
gamma ray. RAPD were done by ten primers that were OPA 9, OPA 10, OPA 13, OPA
18, OPB 2, 3 OPB, OPB 6, 7 OPB, OPB 10, and OPB 11. Data were analyzed using
GenAlex 6.1 Program. The percentage of polymorphic loci of winged bean
population control was 47,54%, while in the 20 and 25 Gy treatment were 62,30%
and 54,10%, respectively. The values of genetic variation based on the
calculation of allele frequeies were 0,236; 0,202 and 0,194 for treatment of 20
Gy, 25 Gy and for control plants, respectively. Meanwhile, the value of genetic
distance ranged from 0.08 to 0.32.
Penulis: A RINI M AESAROH , A DI A MURWANTO , A LICE Y UNIATY
Kode Jurnal: jpbiologidd140844
