IDENTIFIKASI BAKTERI PENGOKSIDASI BESI DAN SULFUR BERDASARKAN GEN 16S rRNA DARI LAHAN TAMBANG TIMAH DI BELITUNG
ABSTRACT: Heavy metals’
contamination disturbs the balance and diversity of microorganism in soil.
Microorganisms that can survive in those conditions are bacteria that capable
of oxidizing heavy metals. Identification based on 16S rRNA was used to determine
characteristics and phylogenetic relationships of bacteria that can oxidize
iron and sulphur in tin mining areas. The aim of this research were to determine
the bacterias characteristics isolated from tin mining areas and the
phylogenetic relation of iron-sulphur oxidizing bacteria on tin mining soil in
Belitung based on 16S rRNA sequences. This research was done using descriptive
method, including isolation, ical characterization, and identification based on
16S rRNA sequences. Morphology characterization included colony and cell morphology
through Gram staining. Molecular characterization included amplification of 16S
rRNA gene (Polymerase Chain Reaction/ PCR), electrophoresis amplicon and
sequencing. Bacteria identification was done by comparing the 16S rRNA gene
sequence in GenBank. The result showed three bacterias were identified by 16S
rRNA have a similarity with Bacillus anthracis strain Ames, Bacillus cereus
ATCC 14579, Staphylococcus sciuri subsp. Sciuri strains DSM 20345 and
Micrococcus luteus NCTC 2665.
Penulis: D HEWANTI PUSPITASARI , HENDRO PRAMONO , OEDJIJONO
Kode Jurnal: jpbiologidd140845
