ISOLASI DAN IDENTIFIKASI BAKTERI TANAH YANG TERDAPAT DI SEKITAR PERAKARAN TANAMAN
ABTRACT: The soil is one
habitat of various bacteria. Rhizosphere microbes in the soil near the roots can
be beneficial to the plants by forming
nodules in some plants Leguminoceae. The bacteria are very helpful in chemical
changes in the soil so that the
chemicals can be absorbed by plants. The purpose of this research was to
determine the type of bacteria that has been found in the soil near and away
from plant roots. Bacteria was isolated from soil near
and away from
the roots at
the level 10-5,
10-6, and 10-7
of dilution, each inoculated in duplicate on media Nutient
Agar (NA) and incubated for 2x24 hours. Grown colonies and colonies that had
thin, beige or transparent, size medium (3mm), and Gram negative characters
were sub-cultured. Selected cultures were suspended in concentration about 108
cells / mL 30-50 mL. The identification used the API 20NE. One strip API 20NE. Identification results
indicated a positive
color change in
the media. The
research obtained bacteria that has been found near plant roots was
Pseudomonas luteola, whereas the bacteria that has been found in away plant
roots soil was P. aureuginosa.
Penulis: Dwi Ratna Sari
Kode Jurnal: jpbiologidd150712
