ISOLASI DAN IDENTIFIKASI SENYAWA STEROID FRAKSI KLOROFORM DARI FRAKSINASI EKSTRAK METANOL DAUN KEREHAU (Callicarpa longifolia Lam.)
Abstrak: Kerehau (Callicarpa
longifolia Lam.) is one of the biodiversity used as traditional medicine by one
of the indigenous tribes of Borneo, called Dayak Tunjung. The purpose of this
study was to determine about toxicity level of the Kerehau leaves (Callicarpa
longifolia Lam.) to larvae shrimps (Artemia Salina Leach) and to determine the
content of the active compounds contained in the Kerehau leaves (Callicarpa
longifolia Lam.). The research was conducted by extracting samples with
methanol and then concentrated by rotary evaporator. Total extract fractionated
with n-hexane and chloroform, and then column chromatography applicated to
chloroform fraction. Phytochemicals and BSLT have been test to Isolates
chromatography results, total extract and chloroform extracts to determine the
level of toxicity, FTIR analysis was also performed to isolate the results of
Chromatography. Based on the results test of phytochemical compounds secondary
metabolites of Kerehau leaves (Callicarpa longifolia Lam.) indicated that the
methanol extract containing steroid compounds, phenolics, and flavonoids.
Chloroform fractions containing flavonoids and steroid compounds. Isolates
Chloroform fraction containing Steroids compounds. On Brine Shrimp Lethality
Test, death of larvae shrimps (Artemia Salina Leach) were recorded and
processed using SAS Probit analysis to determine the value of Lethal
Concentration (LC50) to the value of each is, in chloroform isolates with LC50
values of 96.4096 ppm, chloroform extract had LC50 values of 104.1878 ppm and
methanol extract had LC50 values of 485.1681 ppm. In Chloroform fraction
separation and purification using column chromatography and recrystallization
techniques to produce pure compound that is found in fractions A.f. The
compounds were characterized by FTIR spectrometer spectroscopy. Based on FTIR
analysis of the isolates showed chloroform fraction compound characterization
using IR spectrophotometer is IR λmaks cm -1 : 3248.12; 2862.36; 2939.52;
2607.76; 2345.44; 1458.18; 1496.76; 1597.06; 1172.72; 1226.73; 1288.45;
1658.78; 1095.57; 1002.98, and 941.26. Based on the results obtained isolates
suspected compound is a steroid class of sterols.
Keywords: Kerehau, Callicarpa
longifolia Lam., toxicity test, Phytochemical test, chloroform, and Steroid
Penulis: Arie Novadiana, Erwin,
Subur P Pasaribu
Kode Jurnal: jpkimiadd140640
