ISOLASI, IDENTIFIKASI DAN UJI TOKSISITAS SENYAWA FLAVONOID FRAKSI KLOROFORM DARI DAUN TERAP (ARTOCARPUS ODORATISSIMUS BLANCO)

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Abstrak: The research about the characterization of flavonoid compound from the leaves of plants applicability chloroform (artocarpus odoratissimus Blanco). Extraction plants done by maceration method and separation of compounds was performed using the technique of vakum liquid chromatography and chromatography colomn flash. Separation result obtained yellowish-white powder. Resul spectrum UV-visible absorption obtained namely the 254.37 to 181.28 area absorbance wavelength (nm), while FT-IR analysis result provide absorption at wavenumber there is an alkyl group, a hydroxyl group, an aromatic group and C-O-C stretching based on the data spectrum UV an IR spektrun can be concluded that the compounds suspected isolated belonged to the Flanva-3-ol. Toxicity was conducted for prawn larvae Artemia salina Leach. Effect of toxicity from isolate identified with presentage of prawn larva and counted by probit analysis (LC 50 ). The results of this test showed that the the isolate was very toxic with LC 50 value of 26,8824 ppm. The test result obtained mortality of shrimp larvae LC 50 value of each sample is 110.5176 ppm total extract, chloroform fraction 147.7895 ppm, and isolate 80.2568 ppm.
Keywords: Terap (Artocarpus odoratissimus Blanco), Isolation, Flavonoid, BSLT
Penulis: Nur Tasmin, Erwin, Irawan W Kusuma
Kode Jurnal: jpkimiadd140645

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Jp Kimia dd 2014