PENGGUNAAN BEBERAPA MEDIUM SEMISINTETIK UNTUK PRODUKSI MISELIUM JAMUR MAITAKE (Grifola frondosa (Dickson: Fr.) S. F. Gray) ISOLAT CIANJUR DAN EKSTRAK KASARNYA
ABSTRACT: Research on the use
of some semisynthetic medium for the production of fungal mycelium Maitake
(Grifola frondosa (dickson: Fr.) SF Gray) isolates Cianjur and crude extract
was performed with an incubation period of 30 days. This study aimed to
determine the ability of green bean, cowpea and maize as an alternative
material of semisynthetic medium for manufacturing G. frondosa’s mycelium and
and to know the production of G. frondosa’s mycelium and the highest crude
extract. This study was experimental study with a completely randomized design
consisted of 4 treatments : Yeast Potato Dextrose Broth (PDYB) medium, Green
bean Yeast Dextrose Broth (GbDYB) medium, Cowpea Yeast Dextrose Broth (CpDYB)
medium and Corn Yeast Dextrose Bro(CDYB) medium. The highest average dry weight
of mycelium (1,584 g/100ml) was GbDYB medium. The lowest average dry weight of
the mycelium (g/100ml 0.244) was PDYB medium. The weight of the crude extract
of mycelium in each treatment was lower than the dry weight. The highest weight
of the crude extract was obtained from the GbDYB medium treatment (1,22 g) and
the lowest was obtained from PDYB medium (0,113 g). Anova test results different treatment was very significant,
meaning that the use of extract of green bean, cowpea and maize greatly
affected the growth of G.frondosa’s mycelium. The LSD test between treatment of
PDYB medium and CDYB medium was not significant, meaning that the increase of
mycelium’s growth on PDYB medium had no different with the CDYB medium.
KEYWORDS: cowpea,
extracellular polysaccharides, Grifola frondosa, green beans, maize, mycelium,
semisynthetic medium
Penulis: MARIA MARDHITAMA
MAHARANI, NUANIEK INA RATNANINGTYAS, SLAMET PRIYANTO
Kode Jurnal: jpbiologidd140846
