Sitotoksisitas Ekstrak Aspergillus fumigatus dari Daun Mekai (Albertisia papuana Becc.) terhadap Sel Kanker Payudara T47D dan MCF-7
ABSTRACT: The previous
studies showed that
the Albertisia papuana
Becc. root have cytotoxicity on breast cancer. The A.
papuana root toxicity on breast cancer could not only by
plant secondary metabolism,
but may be
also by secondary
metabolism of endophytes. Aspergillus
fumigatus is one of endophytes
that have anticancer
agent. Endophytes can be
distributed dynamically in
whole of plant
organ, one of
them are leaves. Therefore the
objective of this studies were to know the presence of A. fumigatus in A.
papuana leaves, and the cytotoxicity of their secondary metabolism on breast
cancer cells. The sample of A. papuana were collected from Botanical Zoo of
Bogor, while T47D and MCF-7 cell
lines were obtained
of Tropical Medicine’s
Faculty, UGM. Isolation
of endophytes was done by growing leaves extract on water agar 2 %
medium. Secondary metabolism was extracted from fermented broth using in ethyl
acetat and n-butanol. The cytotoxicity
was perform by
MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide) assay. The result showed that A. fumigatus assosiated with A.
papuana leaves. Ethyl acetat extract from
fermented A. fumigatus both on T47D and MCF-7 cell lines had lower (IC50. 50,
444 µg/ml and 59 µg/ml) than n-butanol (IC50. 103, 398 µg/ml and 127,188 µg/ml. It
could be said
that A. fumigatus
from A. papuana
leaves could induce cytotoxicity on T47D and MCF-7 breast
cancer cells.
Keywords: cancer, Aspergillus
fumigatus, secondary metabolism, Albertisia papuana Becc., cytotoxicity
Penulis: Hasnaul Maritsa,
Soekarti Moeljopawiro, Rina Sri Kasiamdari
Kode Jurnal: jpbiologidd150714
