UJI BIOAKTIVITAS PADA EKSTRAK KASAR ETANOL, FRAKSI n-HEKSAN, ETIL ASETAT DAN ETANOL-AIR DARI DAUN ANDONG (Cordyline terminalis Kunth)
Abstrak: Phytochemical, brine
shrimp lethality and antibacterial activity tests in each fraction from Andong
leaves extract(Cordyline terminalis Kunth) that came fromUnmul Samarinda
botanical garden, Kalimantan Timur has been carried out. The leaf was extracted
with ethanol andconcentrated by using rotary evaporator. The crudeethanol
extract was fractioned with n-hexane, and ethyl acetate. Based on the secondary
metabolites phytochemical test of the Cordyline terminalis Kunth leaves show
that crudeethanol extract is contain phenol, saponin and steroid.n-hexane
fraction iscontain steroid.Ethylacetate fraction is contain phenol and steroid.
And ethanol-water fraction is contain phenol and saponin. Brine shrimp
lethality test exhibit mortality rate of Artemiasalina (L) by Probit SAS
analysis to determine lethal concentration 50% (LC 50 ) value. The test showed
that the most active wascrudeethanol extract with LC 50 value of 26.8788 ppm.
Antibacteria activity test of extracts for Bacillus cereus bacteria
(positiveGram) andEscherichia coli (negativeGram) were carried out by paper
disk method. The test showed that the most active were ethyl acetate extract
with minimum inhibitor consentration of 2-10% which clear zone diameter was 6,4
mm on Bacillus cereus bacteria and 6 mm on Escherichia coli bacteria.
Penulis: Ritson Purba, Enos
Tangke Arung, Teddy Tranoto
Kode Jurnal: jpkimiadd140655
