Uji Efektivitas Metode Isolasi DNA Genom Kopi Arabika (Coffea arabica L.) Asal Kabupaten Jayawijaya
ABSTRACT: Genetic substance
of DNA has
many functions as
a basic component
of the organism.
DNA can be
obtained directly through the
isolation of DNA.
Isolation of genomic
DNA Wamena arabica
coffee is done
by treating the young leaves to get DNA extract. This
reasearch is intended to provide scientific contributions in an effort to
screen the best methods
of DNA isolation,
including a modified
extraction of Zheng
et al method
(2005), a modified method of Doyle and Doyle (1990),
and method of George and Khan modificaation (2008) or CTAB method. All of methods were
tested on Arabica
coffee Jayawijaya Wamena.
Testing was done
by looking at
the difference in quality and quantity of products in the
form of genomic DNA concentration and DNA thickness, comparing with the marker
DNA then electrophoresis on agarose gel. From the results of testing the
effectiveness of three types of isolation methods, it was found that the method
of George and Khan (2008) or CTAB
genomic DNA produce the best quality
than other methods. In terms of quantity, the criteria in the form of DNA
concentrations ranging from 100 ng λ-DNA/l, λ-DNA 50
ng/l, λ-DNA 10 ng/l.
Concentration of genomic
DNA bands visible
when the profile is visualized in
gel electrophoresis with UV luminescence. This study will be a step and initial
information about the genetic
composition of a
population of arabica
coffee which still
exists, and will
be developed through DNA amplification technique.
Penulis: ARSYAM MAWARDI, MARIA
L. SIMONAPENDI
Kode Jurnal: jpbiologidd160002
