UJI FITOKIMIA DAN AKTIVITAS ANTIBAKTERI DARI TANAMAN MERKUBUNG (Macaranga gigantea (Rchb.f. & Zoll) Mull. Arg.) DENGAN EKSTRAK TOTAL, FRAKSI n-HEKSANA, ETIL ASETAT DAN ETANOL AIR TERHADAP BAKTERI Escherichia coli dan Staphylococcus aureus
Abstrak: Phytochemical tests,
mortality of larvae shrimp (brine shrimp lethality test) and the antibacterial
activity of the leaf merkubung (Macaranga gigantea Rchb.f. & Zoll.) With
various factions has been conducted. Merkubung leaf samples were extracted with
ethanol and concentrated by using rotary evaporator. The total extract was
fractionated with n-hexane and ethyl acetate. The results of phytochemical
compounds secondary metabolites merkubung leaves showed that the total extract
are contains alkaloids, phenolics and steroids. n-hexane fraction are
containing alkaloids, flavonoids and steroids. Ethyl acetate faction are
containing flavonoids and steroids. Ethanol-water faction is containing phenolic
compounds. Shrimp larvae mortality trials showed mortality rates of larvae of
Artemia salina (L) by using SAS Probit
analysis to determine the value of Lethal Concentration 50 % (LC50). The test
shows that the most toxic fraction is the total extract with LC50 values of
459.8414 ppm. The test of antibacterial activity using paper disc method and
shows that the most active faction is the total extract with a MIC (Minimmum
Inhibitor Concentration) of 1 % is 6.5 mm diameter clear zone on Staphylococcus
aureus (Gram positive) and 6.25 mm in the bacterium Escherichia coli (Gram
negativ).
Penulis: Azhar Seli Apriani,
Chairul Saleh, Alimuddin
Kode Jurnal: jpkimiadd140644
