UJI FITOKIMIA, TOKSISITAS DAN AKTIVITAS ANTIBAKTERI TERHADAP EKSTRAK ETANOL DAUN RAMBUSA (Passiflora foetida L.) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli
Abstrak: Phytochemical tests
were conducted, brine shrimp lethality test / BSLT and antibacterial activity
test of the coarse extract and fractions from the Rambusa leaves (Passiflora
foetida L.) derived from Samarinda, East Kalimantan. Rambusa leaf samples
(Passifloa foetida L.) was extracted by ethanol, then concentrated using rotary
evaporator. Then fractionated by using n-hexane and ethyl acetate solvent.
According the test results, phytochemical compounds of secondary metabolites
has contained alkaloids and steroidal compounds, triterpenoids. Within the
antibacterial activity test using Staphylococcus aureus (Positive gram) and
Escherichia coli (Negative Gram) using the discs method. This test using
concentration in 1%, 5%, 10%, 15%, 20% resulted that the most active fraction
is the fraction of n-hexane with minimum inhibitory rate was 1% and brine
shrimp lethality test within concentration in 1000; 500; 250; 125; 62.5; 31.25;
15.625; 7,8125 ppm showing larval lethality of Artemia salina (L.) using SAS
probit analysis to determine the value of Lethal Concentration 50% (LC 50 ).
This test resulted that the most active fraction is the fraction of n-hexane
with 133.7473 ppm of LC 50 values .
Keywords: Passiflora foetida
L. , phytochemical test , test antibacterial activity , bacterial and LC 50
Penulis: Noviyanti Y, Subur P
Pasaribu, Daniel T
Kode Jurnal: jpkimiadd140643
