Uji Resistensi Antibiotik dan Deteksi Gen Plasmid IncHI1 Salmonella typhi Isolat Jayapura
ABSTRACT: The research
on antibiotic resistance
test and detection
of gene Plasmid
IncHI1 1 Salmonella
typhi isolates of Jayapura
have been conducted
in January to
August 2015. The
purpose of this
study is to
determine antibiotic resistance
patterns and detect the presence ofS. typhi IncHI1 plasmid genes in
Jayapura.The method of study was the laboratory analytic where ninety two
samples were culture tested and S. typhi
identified was tested using disc diffusion
method of Kirby
Bouer to see
any resistance toward
18 antibiotics. Plasmids
of S. typhi
then were extracted using method
of Kado-Liu. To ensure the presence of S. typhi DNA, the testof flagellin gen
detection had been done using Nested PCR followed by gene Inchi1 S. typhi detection. The results showed that
among 8 isolates of S. typhi,
there has been
a Multi Drug
Resistant (MDR) of
7 samples (87.5%)
with varying resistance
patterns where the most resistant antibiotics are Amoxicillin 100% (8
isolates), Cefazolin 75% (6 isolates), Ampicillin 75% (6 isolates), Trimethoprim–Sulfamethoksazol 62.5%
(5 isolates), Amikacin
62.5% (5 isolates),
Gentamicin 50% (4 isolates)
and Ampisillin–Sulbactam 50%
(4 isolates). The
most sensitive antibiotics
is Meropenem is
87.5% (7 isolates). PCR test
results showed that there were no genes of IncHI1 S. typhi in Jayapura.
Penulis: RINI S. KELANIT, DIRK
Y.P. RUNTUBOI DAN TRI GUNAEDI
Kode Jurnal: jpbiologidd160006
