AKTIVITAS ANTIOKSIDAN DAUN BAWANG MEKAH (Eleutherine americana Merr.) DENGAN METODE DPPH (2,2-difenil-1-pikrilhidrazil)
Abstrak: Antioxidant is a
substance which in small concentrations can significantly inhibit or prevent
the oxidation of the substrate. Antioxidant has an important part in health.
One of plants that have antioxidant activity is bawang mekah (Eleutherine
americana Merr.). This research aims to determine the antioxidant activity of
bawang mekah leaves. Antioxidant activity assays performed using DPPH
(2,2-diphenyl-1-picrylhydrazyl) method, begins with the extraction using
ethanol 70% by maseration. Ethanol fraction performed phytochemical screening
with tube test method and preliminary test with DPPH method by silica gel 60
F254 Thin Layer Chromatography (TLC) eluted by chloroform p.a mobile phase. The
antioxidant activity of fraction measured using UV-Vis Spectrophotometer with
10, 20, 30, 40 μg/mL variation concentration and vitamin C with 2; 2,5; 3; 3,5
μg/mL as a positive control. The results of phytochemical screening showed the
fraction contained flavonoids, phenols and saponins. The result of preliminary
test gained 1 spot with 12,50 hRf visualized with 366nm UV light. The fraction
spot showed positive result of antioxidant test was signed by changed color to
yellow with purple background after sprayed with DPPH 0,2%. The results of
UV-Vis Spectrophotometer measurements showed the ethanol fraction had
antioxidant activity with IC50 45,33694μg/mL and vitamin C had IC50 0,62147
μg/mL.
Penulis: Isnindar
Kode Jurnal: jpfarmasidd140381
