AN LC- MS/MS METHOD FOR THE DETERMINATION OF OMEPRAZOLE ON PROTON PUMP INHIBITOR IN HUMAN PLASMA
Abstract: A sensitive and
selective liquid chromatographic method coupled with tandem mass spectroscopy
(LC-MS/MS) was developed for the quantification of omeprazole in human plasma.
Lansoprazole was used as internal standard with plasma samples, extracted using
10mM ammonium acetate. A centrifuged upper layer was then evaporated and
reconstituted with Acetonitrile: mobile phase buffer 70:30%v/v. The
reconstructed samples were injected into a C18 column purospher star 5µ. The
mobile phase was composed of ACN: mobile phase buffer (5mm ammonium bicarbonate
buffer) in the ratio of 70:30%v/v with flow rate 1.0mL/min. The mass
spectrometer was operated using positive ion mode and turbo electro spray
ionisation. Nitrogen was used as the nebulizer, curtain, collision and
auxiliary gases. Using MS/MS with multiple reactions monitoring (MRM) mode,
omeprazole was detected without severe interferences from plasma matrix.
Detection of omeprazole in human plasma was accurate and precision. This method
has been successfully applied to the study of omeprazole in human specimens
Author: T. Sudha, Kalan Kumar
Reddy, P. V. Hemalatha, V. R. Ravikumar
Journal Code: jpfarmasigg160027
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