PENETAPAN KANDUNGAN FENOLAT TOTAL DAN UJI AKTIVITAS ANTIOKSIDAN MENGGUNAKAN RADIKAL DPPH FRAKSI ETIL ASETAT SARI BUAH APEL BELUDRU (Diospyros blancoi A. DC.)
Abstract: Antioxidant plays a
role in inhibiting oxidation by binding to free radicals. As a result, the cell
damage that leads to degenerative diseases can be inhibited. This research was
conducted to determine the antioxidant activity and total phenolic content of
ethyl acetate fraction of velvet aple (Diospyros blancoi A.DC.) juice.
Previously known that other plants from genus Diospyros contain phenolic
compounds such as quercetin. Antioxidant activity test performed qualitatively
and quantitatively using radical of 1,1-diphenyl-2 pikrilhidrazil (DPPH) and
expressed as the value Inhibition Concentration 50 (IC50). Determination of
total phenolic using Folin-Ciocalteu method expressed as equivalent mass of
gallic acid. Phenolic compounds will be oxidized by the Folin-Ciocalteu under
alkaline conditions, forming a blue solution. The results showed that the ethyl
acetate fraction of velvet apple juice has very strong antioxidant activity
with IC50 of (30.0 ± 0.09) mg / mL. Total phenolic content of (393.5 ± 0.35) mg
gallic acid equivalents per gram of ethyl acetate fraction.
Keywords: antioxidant, velvet
apple (Diospyros blancoi A. DC), ethyl acetate fraction, DPPH, total phenolic
content
Penulis: Johannes Baptista
Yunio Rahmawan, Yohanes Dwiatmaka
Kode Jurnal: jpfarmasidd130313
