UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN SIRSAK (Annona muricata L) PADA TIKUS PUTIH (Rattus norvegicus) DIABETES MELITUS YANG DIINDUKSI ALOKSAN DENGAN PARAMETER MALONDIALDEHID (MDA)
Abstrak: Hyperglycemia in
Diabetes can caused the excessive production of Reactive Oxygen Species (ROS)
in the body that will causes oxidative
stress that tissue damage. Oxidative stress can indicated by increasing
MDA levels. This research aims to know and determine the concentration ethanol
extract of soursop leaves that have antioxidant activity to MDA levels in
diabetic rats. 9 white rats were divided into 3 groups. All rats were induced
by alloxan 110mg/Kg.BB intraperitoneally. Group I was administered with Na.CMC
as a negative control, group II and III were administered with ethanol extract
of soursop leaves 2,5% and 5%. Administration were given orally for 12 days
orally. Examination of blood glucose and MDA levels conducted before and after
the administration. MDA Assay performed with TBA method was based on reaction
of MDA-TBA as measured on a spectrophotometer 532 nm wavelength. The results
were analysized statistically by using randomized block design (RBD) method.
Based on the research has been done, it was showed that the ethanol extract of
soursop leaves can lower blood glucose levels of diabetic rats and extract with
concentration of 5% has potential as an antioxidant activity that can reduce
MDA levels in diabetic rat.
Penulis: Safriani Rahman,
Rachmat Kosman, Inda Rahmaniar
Kode Jurnal: jpfarmasidd140376
