UJI AKTIVITAS ANTIOKSIDAN MENGGUNAKAN RADIKAL 1,1-DIFENIL-2-PIKRILHIDRAZIL (DPPH) DAN PENETAPAN KANDUNGAN FENOLIK TOTAL FRAKSI ETIL ASETAT EKSTRAK ETANOLIK DAUN BENALU (Dendrophthoe pentandra L. Miq.) YANG TUMBUH DI POHON KEPEL (Stelechocarpus burahol (Bl.) Hook. f.)
Abstract: Antioxidant has the
ability to capture free radicals. These free radicals can oxidize nucleic
acids, proteins, lipids or DNA, causing degenerative diseases. Antioxidants
compound found in plants such as phenolic acids, polyphenols and flavanoids
would capture free radicals such as peroxide, hydroperoxide or lipid peroxyl
and also inhibit the oxidative mechanisms that show degenerative diseases.
Quercetin and quercetin 3-o-rhamnosida as constituents of mistletoes (Dendrophthoe
pentandra (L.) Miq.), are able to provide antioxidant activity. Mistletoes
dried and extracted with 70% ethanol, followed by fractionation with ethyl
acetate. Antioxidant activity of ethyl acetate fraction from ethanol extract
was calculated using DPPH method and obtained IC50 values. The results showed
that the ethyl acetate fraction from ethanolic mistletoes leaf extract has
antioxidant activity with IC50 value of (12.57 ± 0.7) mg / mL. The content of
total phenolic (13.76±0.9) mg gallic acid equivalents per gram of ethyl acetate
fraction from ethanol extract of leaves of the mistletoes.
Keywords: Antioxidant,
misletoes leaf, Dendrophthoe pentandra, ethyl acetate fraction, ethanolic
extract, DPPH, total phenolic
Penulis: Willigis Danu Patria,
C.J. Soegihardjo
Kode Jurnal: jpfarmasidd130307
