UJI AKTIVITAS ANTIOKSIDAN MENGGUNAKAN RADIKAL 1,1-DIFENIL-2- PIKRILHIDRAZIL DAN PENETAPAN KANDUNGAN FENOLIK TOTAL FRAKSI ETIL ASETAT EKSTRAK ETANOL BUAH ANGGUR BALI (Vitis vinifera L.)
Abstract: This research was
conducted to determine the antioxidant activity of ethyl acetat fraction of
extract of Balinesse grape (Vitis vinifera L.) using free radical and determine
the total phenolic content. Balinesse grape was extracted with ethanol and then
fractionated using ethyl acetate. Free radical scavenging activity was tested
by measuring the DPPH radical scavenging activity. Total phenolic content was
determined using the Folin-Ciocalteau method measured the concentration of
phenolic content in gallic acid total equivalents using unit's mg/g. The mean
IC50 value for DPPH radical scavenging activity of the ethyl acetat fraction of
ethanolic extract of Balinesse grape was found to be 36.55 ± 0.09 µg/mL. The
phenolic content was ranging from 3.23 ± 0.02 mg gallic acid equivalents per
gram of ethyl acetat fraction of ethanolic extract of Balinesse grape.
Keywords: antioxidants,
Balinesse grape (Vitis vinifera L.), fraction of ethyl acetat, DPPH, total
phenolic content
Penulis: Mikhael Gustandy,
C.J. Soegihardjo
Kode Jurnal: jpfarmasidd130314
