AKTIVITAS ANTIBAKTERI FRAKSI DIKLOROMETAN DAN N-HEKSANA PAKU SISIK NAGA (DRYMOGLOSSUM PILOSELLOIDES (L) PRESL.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN SALMONELLA TYPHI
ABSTRACT: The potential use of
dragon scales have been used by people in Kalimantan as anti-inflammatory
drugs, oral thrush and toothache. Dragon scales (D. Piloselloides) is one of
epiphytic plants in association with other plants. This study aims to determine
the antibacterial activity of the dichloromethane and n-hexane fraction of
dragon scales against S. aureus and S. typhi. The research was conducted from
October to December 2015 in the Pharmaceutical Chemistry Laboratory Faculty of
Medicine, Wood Technology Laboratory Faculty of Forestry, Chemistry Laboratory
and Biology Laboratory Faculty of Mathematics and Natural Sciences, Tanjungpura
University, Pontianak. Dichloromethane and n-hexane fraction were obtained from
the fractionation of methanol extract. Furthermore, both fractions in TLC and
chromatogram profiles were visualized using UV 254 and 336 nm. Antibacterial
test of D. piloselloides fraction was done by paper disc diffusion method. The
concentration used in this study was 0.25; 0.30 and 0.35 g/ml, and as a
comparison, Ciprofloxasin 0.005 mg/ml used as a positive control. Antibacterial
activity test of dichloromethane fraction of dragon scales has only the ability
to inhibit S. aureus, whereas the n-hexane fraction has no antibacterial
activity against S. aureus and S. typhi. TLC test results showed that the
dichloromethane fraction contains flavonoids compounds, while the n-hexane
fraction contains terpenoids compounds.
Penulis: Heriyati Siti
Khotimah, Elvi Rusmiyanto Pancaning Wardoyo
Kode Jurnal: jpbiologidd160096
