ANALISIS KUANTITATIF B-KAROTEN DAN UJI AKTTVITAS KAROTENOID DALAM ALGA COKLAT TURBINARIA DECURRENS
ABSTRACT: One of the
Indonesian marine natural resources, brown alga of Turbinaria decurrens, used
in pharmacy in order of carotenoid pigment as antioxidant. We interested in
analysis B-caroten in Turbinaria decurrens and antioxidant and anti-tumor
activity. The method that is use to divide carotenoid is the hierarchy
maseration method using n-hexane, ethyl acetate
and methanol and then using chromatography column. To analyze
the qualitative carotenoid uses HPLC by
Crc column and eluen methanol-acetonitrile
(3:I). Meanwhile, the test of bioactivity carotene uses
radical DPPH (1I'difenil-2pibilhidrozil) to
test of antioxidant
qnd sitotoksik asscy with MTT
[3,(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrcaolium bromideJ
for HeLa tumor cells to test of anti-tumor. The result of this research is B -
caroten thqt is in qctract has 0.00387%.
Moreover, bioacttvity test
shows that B -caroten fraction does
not active to neutralize of DPPHfree radical than ascorbic
aci{ but it shows
the acttvity to kill HeLa
tumor cells.
Penulis: Fransisca Biranti,
Muhammad Nursid, Bambang Cahyono
Kode Jurnal: jpmatematikadd090083
