EVALUASI AKTIVITAS ANTI-INFLAMASI DAN ANTIOKSIDAN SECARA IN-VITRO, KANDUNGAN FENOLAT DAN FLAVONOID TOTAL PADA TERMINALIA SPP
Abstract: Terminalia is the
second largest genus of Combretaceae family, consists of 200 species
distributed in the tropics and subtropics. Many species of Terminalia are well
kown in traditional medicine in several
countries of Africa and Asia. In this study, bark extracts of four species of
Terminalia (T. catappa, T. citrina, T. bellirica and T. macadamii) were
evaluated for their in-vitro anti-inflammatory and antioxidant activity.
Determination of the anti-inflammatory activity was carried out using red blood
cell membrane stabilization assay and the antioxidant activity was carried out
using DPPH free radical scavenging and ferric reducing power assay. Estimation
of total phenolic content was carried
out using Folin-Ciocalteu reagent, while
total flavonoid content using alumminium trichloride reagent. The
results showed that extract of T. catappa has the most potent anti-inflammatory
activity (IC50= 97,83±0,0100 ?g/mL) and the most powerful antioxidant activity of DPPH free radical
scavenging (IC50= 21,8900 ± 0,0264 ?g/mL) and ferric reducing power (IC50=
87,1533 ± 0,04163 ?g/ mL). The weakest anti-inflammatory activity was T.
citrina extract (IC50= 159,35±0,0200 ?g/mL), whereas T. bellirica extract
demonstrated the weakest antioxidant activity of DPPH free radical scavenging (IC50= 44,4867 ±
0,0153 ?g/mL) and ferric reducing power
(IC50= 140,89±0,04 ?g/mL). The extract of T. citrina has the highest content of
total phenolic (84,8167 ± 0,53407 mg GAE/g) and flavonoid (23,1200 ± 1,7149 mg
RE/g) while the T. macadamii extract has the lowest content of total phenolic
(24,3700 ± 0,0173 mg GAE/g) and flavonoid (13,2667 ± 0,3386 mg RE/g).
Penulis: Tri Murningsih, Ahmad
Fathoni
Kode Jurnal: jpbiologidd160721
